Anti-Chikungunya E1 Protein-Purified?in vivo?PLATINUM? Functional Grade
Product Sizes
1 mg
£650.00
12-8200-1MG
5 mg
£873.00
12-8200-5MG
About this Product
- SKU:
- 12-8200
- Additional Names:
- CHIKV; Chikungunya virus; VLPs; Chikungunya virus-like particles
- translate.label.attr.clone:
- CHK-166
- Clonality:
- Monoclonal
- Extra Details:
- Specificity: CHK-166 activity is directed against CHIKV E1. Antigen Distribution: E1 is expressed on the surface of CHIKV. Background: Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes epidemics globally and has been declared a notable disease by the CDC1;2. CHIKV is an enveloped virus with an 11.8-kb single-stranded; positive-sense RNA genome with two open reading frames3;4. There are three main genotypes; having 95.2 to 99.8% amino acid identity: Asian; West African; and East/Central/South African (ECSA). The mature CHIKV virion is comprised of a nucleocapsid protein C and two glycoproteins; E1 and E25. E1 participates in virus fusion. E2 functions in attachment to cells. E1 and E2 form 80 trimeric spikes on the virus surface6. CHK-166 is a neutralizing monoclonal antibody (MAb) that provides complete protection against lethality as prophylaxis in Ifnar?/? mice5. It was generated by infecting adult Irf7?/? C57BL/6 mice with the La Reunion 2006 OPY-1 strain of CHIKV (CHIKV-LR) and boosting with recombinant CHIKV E2 protein or infectious CHIKV-LR. Myeloma cell-splenocyte fusions were screened for binding to CHIKV-LR infected cells and the resulting MAb was cloned for analysis. Neutralization escape variants were generated to map the CHK-166 epitope5. CHK-166 recognizes amino acids on domain II of E1; adjacent to the conserved fusion loop. All escape mutants had a single K61T mutation in the E1 protein. CHK-166 inhibits CHIKV infection in cell culture in a post-attachment neutralization assay5. CHK-166 also protects 63% of mice from death when a single dose is administered 24 h after CHIKV infection. If both CHK-166 and CHK-152 are administered post-infection in mice; then viral resistance is prevented and the treatment window is extended5. Additionally; combination CHK-152/CHK-166 MAb therapy in rhesus macaques reduces viral infection and spread; neutralizes reservoirs of infectious virus; and does not produce escape viruses7.
- Host:
- Mouse
- Purification:
- Purity: >=98% monomer by analytical SEC Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a mu
- Reactivities:
- Mouse
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- This antibody may be stored sterile as received at 2-8[o]C for up to one month. For longer term storage; aseptically aliquot in working volumes without diluting and store at
- Supplier:
- Abeomics
- Type:
- Antibodies:In Vivo Antibodies
- Manufacturer's Data Sheet:download_pdf.php
