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Antibodies

Phospho-Btk (Tyr551) (Clone: G12) rabbit mAb Biotin conjugate

Product Sizes
10 Tests
£532.00
12-4348-10TESTS
100 Tests
£792.00
12-4348-100TESTS
About this Product
SKU:
12-4348
Additional Names:
Bruton tyrosine kinase; Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; AGMX1; B-cell progenitor kinase; BPK
translate.label.attr.clone:
BtkY551-G12
Clonality:
Monoclonal
Conjugate:
Biotin
Extra Details:
Btk is a major node in the B-cell receptor signaling pathway; where it regulates B cell maturation; activation; survival; differentiation; and proliferation. Btk is activated by Src family kinases; including Lyn; which phosphorylates Btk at Tyr551. Upon phosphorylation at this site; Btk is recruited to the plasma membrane where autophosphorylation at Tyr223 occurs. The Btk signaling pathway is a major target of small molecule inhibitors for B-cell lymphoma; autoimmune diseases; and non-Hodgkin's lymphomas. These inhibitors either form a covalent bond at Cys481 in the ATP-binding site or serve as reversible inhibitors that bind the SH3 pocket and stabilize inactive Btk.
Format:
Conjugated
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding human Btk (Y551)
Isotype:
Rabbit IgG1k
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at 2-8[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.12 mg/mL total protein coated lysate and 1 µg/mL BtkY551-G12-Biotin conjugate IgG.
Fig-3: Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Btk (Tyr551) antibody BtkY551-G12-Biotin conjugate at 0.1 µg/mL.
Fig-3: Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Btk (Tyr551) antibody BtkY551-G12-Biotin conjugate at 0.1 µg/mL.