Phospho-PKC (pan) (Clone: gamma Thr514) (Clone: PF4) rabbit mAb FITC conjugate
Product Sizes
10 Tests
£532.00
12-4342-10TESTS
100 Tests
£792.00
12-4342-100TESTS
About this Product
- SKU:
- 12-4342
- Additional Names:
- Protein kinase C gamma type; PRKCG; PKCG
- translate.label.attr.clone:
- PKCgT514-PF4
- Clonality:
- Monoclonal
- Conjugate:
- FITC
- Extra Details:
- Protein Kinase (PKC) is a 12 member family of serine/threonine kinases termed conventional or classical( a b;g); novel ( d ; e ;and q ); atypical ( z ; l) and PKN and PKC-related (PKN1; PKN2 and PKN3) playing significant role in several signaling processes involved in physiological and pathological setting (1). PKC activation translates into gene expression modulation; cell division; migration; proliferation; differentiation; and cell survival and apoptosis (2).PKC members are classified based on their distinct cofactor requirements and the extent of homology between their regulatory elements (3;4). PKCalpha;beta I;beta II; andgamma constitute the conventional PKC isoforms; characterized by the presence of two cysteine-rich zinc finger domains; C1a and C1b (5;6) which bind to diacylglycerol (DAG) and phosphatidylserine (PS) (7). In addition; PKC contains a C2 domain; responsible for binding anionic phospholipids like phosphatidylinositol bisphosphate (PIP2) in a Ca 2+ -dependent manner (8;9). The atypical PKC; PKCzandlshare ATP-binding domain and the catalytic domain with PKC. They contain a single C1 domain that lacks residues necessary for binding DAG (10). Association of PKCg to the membrane enables a conformation that permits phosphoinositide-dependent protein kinase1 (PDK-1) (11) to bind and phosphorylate a site in the activation loop; Thr514. (12;13). Thr514 phosphorylation leads to a conformation change enabling phosphorylation of at two carboxyl-terminal sites namely; the turn motif and hydrophobic motif; as a result of which the fully phosphorylated conventional PKC is released from the membrane; and positioned in the cytoplasm as an inactive form (14;15). Binding of Ca 2+ induces low-affinity interaction with the membrane; whereas the membrane imbedded cofactor DAG to PKC results in high-affinity interaction of PKC with the membrane (16).
- Format:
- Conjugated
- Host:
- Rabbit
- Immunogen:
- A synthetic phospho-peptide corresponding to residues surrounding Thr514 of human phospho PKCg
- Isotype:
- Rabbit IgG1k
- Reactivities:
- Human, Mouse, Rat
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Store at 2-8[o]C. Avoid repeated freeze and thaw cycles.
- Supplier:
- Abeomics
- Type:
- Antibodies:Monoclonal Antibody
- Manufacturer's Data Sheet:1.jpg
