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Antibodies

Phospho-MCM2 (Ser139) (Clone: B12) rabbit mAb

Product Sizes
20 µl
£631.00
12-4329-20UL
200 µl
£1021.00
12-4329-200UL
About this Product
SKU:
12-4329
Additional Names:
DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; CCNL1; CDCL1; KIAA0030
translate.label.attr.clone:
MCM2S139-B12
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
The members of minichromosome maintenance (McM) protein family 2-7 were originally identified as a group of proteins essential for DNA replication (chromosomal maintenance (1;2). They share common sequence homology to each other in their nulceotide-binding domains and are distinct subgroup of the large AAA ATPase family; which are required for the initiation and elongation of DNA replication. It has been reported that Cdc7/Dbf4 phospohrylates MCM2 during G1/S cell cycle which coincides with the intiation of DNA replication (3;4)
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho MCM2
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Western blot analysis of L929 cell extract untreated or treated with 25uM etoposide using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.05 µg/mL.
Fig-3: Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.1 µg/mL.
Fig-4: Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 µg/mL Phospho-MCM2 (Ser139) antibody MCM2S139-B12. Company C antibody at 0.07 µg/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-4: Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 µg/mL Phospho-MCM2 (Ser139) antibody MCM2S139-B12. Company C antibody at 0.07 µg/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-5: Flow cytometric analysis of A431 cells; secondary antibody only negative control (blue); or untreated (grey) or treated with staurosporine (orange) using 0.01 µg/mL isotype control or untreated (red) or treated (green) using Phospho-MCM2 (Ser139) antibody MCM2S139-B12 at 0.01 µg/mL.