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Antibodies

Phospho-S6-Ribosomal Protein (Ser240/244) (Clone: CD10) rabbit mAb

Product Sizes
20 µl
£631.00
12-4291-20UL
200 µl
£1021.00
12-4291-200UL
About this Product
SKU:
12-4291
Additional Names:
40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein eS6; RPS6
translate.label.attr.clone:
S6RPS240244-CD10
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
Ribosomal protein S6 kinase is one of two parallel signaling pathways downstream of mTOR; with the other being 4E-BP1. mTOR phosphorylates and activates S6 kinase; which then phosphorylates ribosomal protein S6. The pathway regulates cell growth and cell cycle progression. The identified phosphorylation sites of S6 are Ser235; Ser236; Ser240; Ser244; and Ser247; which are evolutionarily conserved in higher eukaryotes. Ser236 has been proposed as the primary phosphorylation site. Studies using S6 knockin mice; where all five phosphorylation site serine residues are replaced by alanine; have provided extensive detail on S6 function. These studies support the role phosphorylated S6 plays in regulation of cell size; glucose homeostasis; and protein synthesis.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser240/244 of human phospho S6 Ribosomal protein
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Western blot analysis of NIH3T3 cells untreated or treated with PDGF using S6-Ribosomal Protein (S240/244) antibody S6RPS240/S244-CD10 at 10 ng/mL.
Fig-3: Flow cytometric analysis of K562 cells; unstained untreated cells as negative control (light blue) or stained untreated (red) or treated with EGF (green) or stained untreated and blocked with non-phosphophopetide (blue) or phosphor-peptide (black) or stained treated and blocked with non-phospho-petide (violet) or treated and blocked with phosphor-peptide (light green) using Phospho-S6 ribosomal protein (Ser240/Ser244) antibody S6S240S244-CD10 at 0.1 µg/mL.
Fig-4: Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF + pervanadate (green) using 0.1µg/mL Phospho-S6 ribosomal protein (Ser240/244) antibody S6S240S244-CD10 or Company C antibody at 0.4µg/mL (manufacturer's recommended concentration).
Fig-4: Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF + pervanadate (green) using 0.1µg/mL Phospho-S6 ribosomal protein (Ser240/244) antibody S6S240S244-CD10 or Company C antibody at 0.4µg/mL (manufacturer's recommended concentration).