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Antibodies

Phospho-SEK1/MKK4 (Ser257) (Clone: C5) rabbit mAb

Product Sizes
20 µl
£631.00
12-4290-20UL
200 µl
£1021.00
12-4290-200UL
About this Product
SKU:
12-4290
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK4; JNK-activating kinase 1; MEK4; SAPKK1; JNKK1; PRKMK4; MAP2K4; SERK1; SKK1
translate.label.attr.clone:
SEK1MKK4S257-C5
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
SEK1; in cooperation with MKK7; activates JNK by tyrosine phosphorylation in response to cellular stress signals; including osmotic stress; DNA damage; cytokines; and heat shock. SEK1 itself is activated by serine phosphorylation by MAP3Ks; including MEKK1; MLKs; ASK1; and TAK1. In response to TNF alpha stimulation in melanoma cells; filamin interacts with SEK1 as a scaffold protein. SEK1 plays an important role in T cell signaling pathways. Both SEK1-dependent and independent pathways operate for SAPK activation in response to different cellular stresses and stimuli. SEK1 also plays an important role in embryonic development. SEK1 may activate c-Jun and c-Fos during mammalian embryonic liver development.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser257 of human phospho SEK1/MKK4
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.1 µg/mL.
Fig-3: Western blot analysis of THP1 cell extract untreated or treated with sorbitol using 0.05 µg/mL Phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 or Company C antibody at 0.01 µg/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-4: Western blot analysis of COS7 cell extract; untreated or treated with UV plus TPA using phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.05 µg/mL.
Fig-4: Western blot analysis of COS7 cell extract; untreated or treated with UV plus TPA using phospho-SEK1/MKK4 (Ser257) antibody SEK1MKK4S257-C5 at 0.05 µg/mL.