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Antibodies

Phospho-PKCa (Thr497) (Clone: F1) rabbit mAb

Product Sizes
20 µl
£631.00
12-4268-20UL
200 µl
£1021.00
12-4268-200UL
About this Product
SKU:
12-4268
Additional Names:
Protein kinase C alpha type; PKC-alpha; PRKCA; PKCA; PRKACA
translate.label.attr.clone:
PKCaT497-F1
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
PKC alpha is a calcium-dependent isozyme of the PKC family that phosphorylates serine/threonine residues in apoptosis and cellular proliferation and differentiation pathways; including the MAPK cascade. PKC alpha directly phosphorylated Raf-1; inducing survival genes. An increase in PKC alpha is associated with multi-drug resistance in cancer cell lines; and increased expression in breast cancers is noted as causing a particularly malignant phenotype. Thus PKC alpha has been the target of novel cancer therapeutics; with some promising developments in microRNA inhibitors. PKC alpha is itself phosphorylated by mTOR. PKC alpha also plays an important role in water regulator and solute absorption in the cell; where it regulates aquaporin 2 by initiating AQP2 ubiquination and lysosomal degradation.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Thr497 of human phospho PKC alpha
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Western blot analysis of HT1080 cell extract; untreated or treated with TPA using 0.05 µg/mL Phospho-PKCa (Thr497) antibody AWBPKCAT497-F1.
Fig-3: Western blot analysis of HT1080 cell extract untreated or treated with TPA using 0.05 µg/mL Phospho-PKC? (Thr497) antibody PKCaT497-F1 or Company A antibody at 1 µg/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-4: Flow cytometric analysis of 3T3 cells; secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 µg/mL isotype control or imatinib (red) or pervanadate (green) using PKC? (T497) antibody PKCaT497-F1 at 0.1µg/mL.
Fig-4: Flow cytometric analysis of 3T3 cells; secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 µg/mL isotype control or imatinib (red) or pervanadate (green) using PKC? (T497) antibody PKCaT497-F1 at 0.1µg/mL.
Fig-5: Western blot analysis of C6 cell extract treated with Anisomycin using 0.1 µg/mL PKC? (T497) antibody PKCaT497-F1.