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Antibodies

Phospho-Lck (Tyr505) (Clone: A3) rabbit mAb

Product Sizes
20 µl
£631.00
12-4137-20UL
200 µl
£1021.00
12-4137-200UL
About this Product
SKU:
12-4137
Additional Names:
Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Protein YT16; T cell-specific protein-tyrosine kinase
translate.label.attr.clone:
LckY505-A3
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
Lck is a member of the Src family of non-receptor tyrosine kinases and plays a major role in T cell activation. Lck activates many downstream signaling pathways including Akt/mTOR; SAPK/JNK; PLCg1; and RAS/MAPK. Phosphorylation of Lck at Tyr394 in the catalytic domain at the ATP-binding site stabilizes the open and active form; while phosphorylation at Tyr505 in the C-terminal domain promotes the closed; inactive conformation. Multiple small-molecule drugs used to treat leukemia have been shown to target inhibition of Lck; including imatinib and dasatinib. Lck is thus a promising target for suppressing T-cell responses for the treatment of inflammatory diseases or after organ transplantation.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr505 of human phospho Lck
Isotype:
Rabbit IgG1k
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Western blot analysis of Jurkat cell extract untreated or treated with 200nM calyculin A for 30min using Phospho-Lck (Tyr505) antibody LckY505-A3 at 0.1 µg/mL.
Fig-3: Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Lck (Tyr505) antibody LckY505-A3 at 1 µg/mL.
Fig-4: Western blot analysis of Jurkat cell extract; untreated or treated with calyculin A using 0.1 µg/mL Phospho-Lck (Tyr505) antibody LckY505-A3 or Company C antibody at 1 µg/mL (manufacturer's recommended concentration) developed using the same exposure.
Fig-4: Western blot analysis of Jurkat cell extract; untreated or treated with calyculin A using 0.1 µg/mL Phospho-Lck (Tyr505) antibody LckY505-A3 or Company C antibody at 1 µg/mL (manufacturer's recommended concentration) developed using the same exposure.