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Antibodies

Phospho-Stat4 (Tyr693) (Clone: F6) rabbit mAb

Product Sizes
20 µl
£631.00
12-4133-20UL
200 µl
£1021.00
12-4133-200UL
About this Product
SKU:
12-4133
Additional Names:
Signal transducer and activator of transcription 4
translate.label.attr.clone:
Stat4Y693-F6
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
In response to IL-12 binding; the IL-12 receptor activates the Jak kinases; which phosphorylate tyrosine residues of IL-12RB2. These phosphorylated receptors recruit Stat4 through its SH2 domain; whereupon Stat4 is phosphorylated at Tyr693 in its C-terminal transactivation domain. Phosphorylation promotes Stat4 homodimerization and translocation to the nucleus; where it promotes gene transcription. The N-terminal domain of Stat4 appears to be required for maximal stabilization and for the binding of Stat4 dimers to lower-affinity DNA binding sites. Stat4-deficient mice have demonstrated that this gene is required to both promote Th1 development and inhibit Th2 differentiation due to disabling IL-12 receptor-mediated responses.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr693 of human phospho Stat4
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or untreated (grey) or IFNa + IL-4 + pervanadate-treated (orange) using 0.1 µg/mL isotype control or untreated (red) or treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat4 (T
Fig-3: Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 µg/mL of Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 or Company C at 0.1 µg/mL (manufacturer's recommended concentration).
Fig-4: Flow cytometric analysis of K562 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib and stained (red) or treated with IFNa + IL-4 + pervanadate and stained (green) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.01 µg/mL.
Fig-4: Flow cytometric analysis of K562 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib and stained (red) or treated with IFNa + IL-4 + pervanadate and stained (green) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.01 µg/mL.