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Antibodies

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (Clone: D3) rabbit mAb

Product Sizes
20 µl
£631.00
12-4117-20UL
200 µl
£1021.00
12-4117-200UL
About this Product
SKU:
12-4117
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPKK 3; MAPK/ERK kinase 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; SAPKK2; MAP2K3; MEK3; PRKMK3; SKK2
translate.label.attr.clone:
MKK3S189MKK6S207-D3
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
MKK3 and MKK6 are closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). Phospho MKK3 and phospho MKK6 both phosphorylate and activate p38. p38 phosphorylation dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4;5). Phospho MKK3 and phospho MKK6 activation occurs through phosphorylation at S189 and T222 on MKK3 (2) and S207 and T211 on MKK6 (4;5).
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6.
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 0.1µg/mL.
Fig-3: MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 (green).
Fig-4: Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 µg/mL Phospho-MKK3 (Ser189)MKK6(S207) antibody MKK3S189MKK6S207-D3.
Fig-4: Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 µg/mL Phospho-MKK3 (Ser189)MKK6(S207) antibody MKK3S189MKK6S207-D3.