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Antibodies

Phospho-Stat1 (Tyr701) (Clone: 3E6) rabbit mAb

Product Sizes
20 µl
£631.00
12-4106-20UL
200 µl
£1021.00
12-4106-200UL
About this Product
SKU:
12-4106
Additional Names:
Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84
translate.label.attr.clone:
Stat1Y701-3E6
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
Stat1 mediates the cellular response to IFN alpha; IFNB; and IFNg for the regulation of cell growth and the defense against viral and immune challenges. The Jak-Stat pathway plays a central role in the IFNg response; where Stat1 phosphorylation on Tyr701 causes homodimerization through its SH2 domain; translocation to the nucleus; and binding to gamma-activated sequence (GAS) elements. Early in the activation sequence; Stat1 is also phosphorylated at Ser727 through a mechanism involving PI3 kinase and Akt. Stat1 has been found to correlate with increased resistance to chemotherapeutic drugs. However; Stat1 activation of the immune system helps suppress tumor growth; and multiple melanomas and squamous-cell carcinomas have been known to downregulate Stat1 expression to evade immune surveillance.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr701 of human phospho Stat1
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNa IL-4 and pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1µg/mL.
Fig-3: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (Cat# 12-4086) (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1 µg/mL.
Fig-4: Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using 0.1 µg/mL of Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 or Company C antibody at 0.2 µg/mL (manufacturer's recommended concentration).
Fig-4: Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using 0.1 µg/mL of Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 or Company C antibody at 0.2 µg/mL (manufacturer's recommended concentration).