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Antibodies

Phospho-RelB (Ser552) (Clone: A7) rabbit mAb

Product Sizes
20 µl
£631.00
12-4100-20UL
200 µl
£1021.00
12-4100-200UL
About this Product
SKU:
12-4100
Additional Names:
I-Rel
translate.label.attr.clone:
RelBS552-A7
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
RelB contains the Rel homology Domain (RHD) shared by all members of the NF-kB family (1). It is best known for its roles in lymphoid development; DC biology; and noncanonical signaling (2). RelB is a major contributor to chromatin biology; frequently functioning as a dual transcription factor that silences sets of genes by generating silent facultative heterochromatin and activates euchromatin of others (3). RelB is required to repress immediate-response proinflammatory genes during endotoxin tolerance (4). The N-terminal leucine zipper motif of RelB; a motif unique among the NF-kB family; may associate with more diverse DNA sequences than other NF-kB members (5). RelB binds to DNA but only after forming a heterodimer with NF-kB p50 or p52 (6). Like all NF-kB members; RelB contains an approximately 300-residue region known as the RHD. This region supports many of the NF-kB essential functions; such as DNA binding; dimerization; and nuclear localization (7). RelB; unlike other NF-kB members; has an N-terminal leucine zipper motif (1); a domain that can typically interact with many proteins (8). p100; the C-terminus of NF-kB2 acts as a RelB inhibitor (9); where it sequesters RelB in the cytosol to repress its activity. RelB is phosphorylated rapidly at threonine 84 and serine 552; causing cleavage at the N-terminus and its degradation by the proteasome (10). These phosphorylations are mediated by glycogen synthase kinase-3B; specific inhibition of which blocks phospho RelB phosphorylation and degradation (11). This degradation does not occur when phospho RelB is phosphorylated at serine 368; allowing its association with and stabilization by p100 (12).
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser552 of human phospho RelB
Isotype:
Rabbit IgG1k
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Peptide blocking flow cytometric analysis of 3T3 cells isotype antibody-stained negative control (light blue) or untreated (red) or treated with UV and PMA (green) or untreated and blocked with phospho-peptide (black) or UV/PMA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/PMA and blocked with non-phospho peptide (purple) using Phospho-RelB (Ser552) antibody RelBS552-A7 0.01µg/mL.
Fig-3: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using 0.01 µg/mL Phospho-RelB (Ser552) antibody RelBS552-A7.
Fig-3: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using 0.01 µg/mL Phospho-RelB (Ser552) antibody RelBS552-A7.