Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Phospho-MAPKAPK2 (Thr334) (Clone: H2) rabbit mAb

Product Sizes
20 µl
£631.00
12-4090-20UL
200 µl
£1021.00
12-4090-200UL
About this Product
SKU:
12-4090
Additional Names:
MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAPK-2; MK2
translate.label.attr.clone:
MAPKAPK2T334-H2
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (phospho MAPKAPK-2 or phospho MK2) is phosphorylated and activated by the p38 MAPK and it is known to transduce a range of extracellular signals that result in inflammatory response; cell division and differentiation; apoptosis; and cell motility (1;2). p38 MAPK through MK2 regulates biosynthesis of tumor necrosis factor alpha (TNF alpha) and other cytokines (3). In addition; MK2 is involved with phosphorylating of heat shock protein 27 (Hsp27)(4); pointing to prominent role for MK2 in cancer promotion. MK2 is also activated after DNA damage (5;6); resulting in cell cycle arrest such that cells have the capacity to repair their DNA and continue to proliferate. p38 MAPK phosphorylate MK2 in response to stress stimuli at Thr222; Ser272 and Thr334 (7). Thr334 phosphorylation serves as a switch for phospho MAPKAPK-2 nuclear import and export. In resting cells; p38 MAPK and MK2 form a complex in the nucleus (7). Cellular stress causes the phosphorylation of p38 MAPK by upstream kinases; such as MAPK kinase 3. The activated p38 MAPK then phosphorylates MK2 at Thr222; Ser272; and/or Thr334. When activated at Thr334; both p38 MAPK and MK2 translocate to the cytoplasm. Phosphorylation at Thr222 within the activation loop is crucial for MK2-dependent activation of several target substrates; including enzymes; proteins that regulate cytoskeleton motility; mRNA-binding proteins; and regulators of the cell cycle and apoptosis (8).
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Thr334 of human phospho MAPKAPK-2.
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) treated with CalA (green) with 10X non-phospho peptide (violet) or phospho-peptide (brown) using Phospho-MAPKAPK2 (T334) antibody MAPKAPK2T334-H2 0.1 µg/mL.
Fig-3: Western blot analysis of Hela cell extract untreated or treated with UV +TPA using 0.05 µg/mL Phospho-MAPKAPK2 (Thr334) antibody MAPKAPK2T334-H2.
Fig-4: Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or untreated with 0.1 µg/mL of isotype control (orange) or untreated (red) or UV and PMA-treated (green) using Phospho-MAPKAPK2 (Thr334) antibody MAPKAPK2T334-H2.
Fig-4: Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or untreated with 0.1 µg/mL of isotype control (orange) or untreated (red) or UV and PMA-treated (green) using Phospho-MAPKAPK2 (Thr334) antibody MAPKAPK2T334-H2.
Fig-5: Western blot analysis of HeLa cell extract untreated or treated with UV+TPA using 0.05 µg/mL Phospho-MAPKAPK-2 (Thr334) antibody MAPKAPK2T334-H2 or Company C antibody at 0.1 µg/mL (manufacturer's recommended concentration).