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Antibodies

Phospho-SLP-76 (Tyr128) (Clone: 3F8) rabbit mAb

Product Sizes
20 µl
£631.00
12-4084-20UL
200 µl
£1021.00
12-4084-200UL
About this Product
SKU:
12-4084
Additional Names:
Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP76; LCP2
translate.label.attr.clone:
SLP76Y128-3F8
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
SH2 Domain-Containing Leukocyte Protein Of 76 KDa (SLP-76) is an adaptor protein that plays a role in signal transduction in T cells. Studies using a SLP-76-deficient T cell line have demonstrated that SLP-76 is required for optimal phosphorylation and activation of both PLCg1 and the Ras pathway. SLP-76 phosphorylation is mediated by Zap70 upon TCR stimulation. Within an N-terminal acidic region; SLP-76 possesses three tyrosines (Tyr113; 128; and 145); which are phosphorylated upon activation. The sterile alpha-motif (SAM) domain of SLP-76 drives formation of dimers and higher order oligomers. SLP-76 micro-clusters at the immunological synapse enhance signal transduction and T cell activation.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr128 of human phospho SLP-76
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 at 0.01µg/mL.
Fig-3: SLP76Y128-3F8 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 (green).
Fig-4: Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 10 ng/mL of isotype control or untreated (red) or pervanadate (green) using 10 ng/mL of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 or Company B antibody at 10 ng/mL.
Fig-4: Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 10 ng/mL of isotype control or untreated (red) or pervanadate (green) using 10 ng/mL of Phospho-SLP-76 (Tyr128) antibody SLP76Y128-3F8 or Company B antibody at 10 ng/mL.