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Antibodies

Phospho-CrkL (Tyr207) (Clone: G4) rabbit mAb

Product Sizes
20 µl
£631.00
12-4073-20UL
200 µl
£1021.00
12-4073-200UL
About this Product
SKU:
12-4073
Additional Names:
Crk-like protein; CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like
translate.label.attr.clone:
CrkLY207-G4
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
CrkL (v-Crk sarcoma virus CT10 oncogene-like protein) is an adaptor protein composed of one Src Homology 2 (SH2) and two Src Homology 3 (SH3) domains separated by flexible linker sequences that act as building blocks to assemble multiprotein complexes (1). The Crk adaptor proteins (Crk and CrkL) constitute an integral part of a network of essential signal transduction pathways in humans and other organisms that act as major convergence points in tyrosine kinase signaling. CRKL is required for the normal development of multiple tissues that rely on fibroblast growth factor 8 (FGF8). Phosphorylation of Crk on Tyr 221 or CrkL on Tyr 207 causes intramolecular binding of the linker region to the SH2 domain; sequestering the SH2 and SH3N and preventing them from binding target proteins (2;3). Mounting evidence indicates that dysregulation of Crk proteins is associated with human diseases; including cancer and susceptibility to pathogen infections.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Western blot analysis of K562 cell extract untreated or treated with imatinib using 1ng/mL CrkL (Tyr207) antibody CrkLY207-G4.
Fig-3: Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-CrkL (Tyr207) antibody CrkLY207-G4 at 0.01µg/mL.
Fig-4: Western blot analysis of NIH3T3 cell extract using 1µg/mL Phospho-CrkL (Tyr207) antibody CrkLY207-G4.
Fig-4: Western blot analysis of NIH3T3 cell extract using 1µg/mL Phospho-CrkL (Tyr207) antibody CrkLY207-G4.
Fig-5: Western blot of E10.5 mouse wild-type (+/+) or Crkl knock out (-/-) whole embryos. The red channel was stained using a B-actin loading control and the green channel was stained using 1:500 dilution of Phospho-CrkL (Tyr207) antibody CrkLY207-G4. Phospho CrkL antibody staining is absent in the knock out lysate.