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Antibodies

Phospho-ATF2 (Thr71) (Clone: G3) rabbit mAb

Product Sizes
20 µl
£631.00
12-4051-20UL
200 µl
£1021.00
12-4051-200UL
About this Product
SKU:
12-4051
Additional Names:
Cyclic AMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; HB16; CREB2; CREBP1
translate.label.attr.clone:
ATF2T71-G3
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
Activating transcription factor 2 (ATF2); known as cyclic AMP (cAMP) response element (CRE) binding protein 2 (CREB2) and CRE-BP1 is a member of the activating protein-1 (AP1) transcription factor family. Through homo-dimerization or hetero-dimerization with other AP1 family members; such as the CREB; Fos; Maf; or Jun family transcription factor; it regulates the expression of many genes. ATF2 is an important mediator of mammalian cell responses to various stimuli; including stress. As a transcription factor; ATF2 acts as transducing extracellular signals to the nucleus to facilitate transcriptional responses to stimuli. ATF2 is stimulated by growth factors; ultraviolet (UV) radiation; and cytokines. Stress-activated protein kinases (SAPKs)(e.g. p38 MAP Kinase) induce transcriptional activation of ATF2. ATF2 is phosphorylated at amino acids threonine-69 and threonine-71 (T69; T71). Phosphorylation at these threonine residues is essential for maximal transcriptional activity downstream of insulin/epidermal growth factor stimulation and is dependent on cooperation between c-Jun N-terminal kinase (JNK) and signaling cascades downstream of Ras. Upon T69/T71 phosphorylation; ATF2 interacts with other AP1 proteins and translocate to the nucleus to regulate the expression of hundreds of genes.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human phospho ATF2
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 µg/mL Phospho-ATF-2 (Thr71) antibody ATF2T71-G3.
Fig-3: Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL.
Fig-4: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL.
Fig-4: Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL.
Fig-5: Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (T71) antibody ATF2T71-G3 or Company C antibody at 0.07 µg/mL (manufacturer's recommended concentration).