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Antibodies

Phospho-Histone H3 (Ser28) (Clone: D6) rabbit mAb

Product Sizes
20 µl
£631.00
12-4046-20UL
200 µl
£1021.00
12-4046-200UL
About this Product
SKU:
12-4046
Additional Names:
Histone H3.1t; H3t; H3FT; HIST3H3
translate.label.attr.clone:
HisH3S28-D6
Clonality:
Monoclonal
Conjugate:
Unconjugated
Extra Details:
Histones are highly conserved proteins that serve the core of nucleosomes; which serve to organize chromatin fiber for DNA packing. Histone H3 phosphorylation plays a major role in both transcriptional activation; which requires unpacking of the chromatin structure; and in chromosome packing during cell division. Histone H3 is phosphorylated at residues Ser10 and Ser28; and is acetylated at Lys14. Phosphorylation at Ser10 occurs during entry into mitosis prior to chromatin condensation; and phosphorylation at Ser28 follows a similar pattern. In response to EGF stimulation; it has been proposed that sequential Ser10 phosphorylation; then Lys14 acetylation occurs; causing a change in chromatin structure and gene activation.
Format:
Purified
Host:
Rabbit
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3
Isotype:
Rabbit IgG1k
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20[o]C. Avoid repeated freeze and thaw cycles.
Supplier:
Abeomics
Type:
Antibodies:Monoclonal Antibody
  • Manufacturer's Data Sheet:1.jpg
Fig 2 : Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1µg/mL.
Fig-3: Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 µg/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6.
Fig-4: Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.05µg/mL or Company C antibody at 0.2µg/mL (manufacturer's recommended concentration).
Fig-4: Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.05µg/mL or Company C antibody at 0.2µg/mL (manufacturer's recommended concentration).
Fig-5: Western blot analysis of HeLa cells untreated or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.005µg/mL or Company C antibody at 0.07µg/mL (manufacturer's recommended concentration) developed using the same exposure.