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Proteins and Peptides

Recombinant Serratia marcescens Nuclease

Product Sizes
50 KU
£211.00
RPT0008LQ-50KU
100 KU
£306.00
RPT0008LQ-100KU
About this Product
SKU:
RPT0008LQ
Additional Names:
Endonuclease|nuc|nucA|Nuclease
Extra Details:
Recombinant Serratia marcescens Nuclease Protein is produced by Pichia expression system. The target protein is expressed with sequence (Asp22-Asn266) of serratia marcescens Nuclease (Accession #WP_015377376.1) fused with no additional amino acid.
Formulation:
Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Immunogen:
Asp22-Asn266
Molecular Weight:
26.71 kDa
Purity:
≥95%
Shipping Conditions:
Dry Ice
Storage Conditions:
-20[o]C Aliquot. Avoid freeze/thaw cycles., -70[o]C Aliquot. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
Recombinant Serratia marcescens Nuclease was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
One unit (U) is defined as the amount of enzyme required to change the absorption value of A260 by 1.0 (equivalent to complete digestion of 37 ug of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/uL.
Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 ug plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.