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Proteins and Peptides

Recombinant Human VEGF-A/VEGF165 Protein

Product Sizes
10 ug
£145.00
RP01150-10UG
20 ug
£193.00
RP01150-20UG
50 ug
£288.00
RP01150-50UG
100 ug
£431.00
RP01150-100UG
About this Product
SKU:
RP01150
Additional Names:
VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A,MVCD1,VEGF,VPF,L VEGFA,VEGF A
Extra Details:
Recombinant Human VEGF-A/VEGF165 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg191) of human VEGF165 (Accession #NP_001165097.1) fused with a 6xHis tag at the N-terminus.
Formulation:
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Immunogen:
Ala27-Arg191
Molecular Weight:
20.01 kDa
Physical State:
Lyophilized
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 %
Sequence:
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C reconstituted. Avoid freeze/thaw cycles., 2-8[o]C reconstituted., -20[o]C/-70[o]C lyophilized. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Recombinant Human VEGF-A/VEGF165 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
The purity of human VEGF165 Protein (Cat.RP01150) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant Human VEGF165 at 1 ug/mL (100 uL/well) can bind Recombinant Human VEGFR2 with a linear range of 8-20 ng/mL.
Immobilized Recombinant Human VEGF165 at 2 ug/mL (100 uL/well) can bind Human KDR with a linear range of 0.2-11.6 ng/mL.
Recombinant Human VEGF165 stimulates cell proliferation of the human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.19-0.78 ng/mL, corresponding to a specific activity of 1.28 x 106~5.26x 106 units/mg.