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Proteins and Peptides

Recombinant Mouse TNF-alpha Protein

Product Sizes
10 ug
£145.00
RP01071-10UG
20 ug
£193.00
RP01071-20UG
50 ug
£288.00
RP01071-50UG
100 ug
£431.00
RP01071-100UG
About this Product
SKU:
RP01071
Additional Names:
DIF, Tnfa, TNF-a, TNFSF2, Tnlg1f, Tnfsf1a, TNFalpha, TNF-alpha,TNF
Extra Details:
Recombinant Mouse TNF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu80-Leu235) of mouse TNF-alpha (Accession #NP_038721.1) fused with a 6xHis tag at the C-terminus.
Formulation:
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Immunogen:
Leu80-Leu235
Molecular Weight:
18.10 kDa
Physical State:
Lyophilized
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 %
Sequence:
LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C reconstituted. Avoid freeze/thaw cycles., 2-8[o]C reconstituted., -20[o]C/-70[o]C lyophilized. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Recombinant Mouse TNF-alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Mouse TNF-alpha Protein (Cat.RP01071) was greater than 95% as determined by SEC-HPLC.
Immobilized recombinant Mouse TNFA Alpha at 2 ug/mL (100 uL/well) can bind Human TNFRSF1A with a linear range of 4-53 ng/mL.
Immobilized Recombinant Mouse TNF-A Alpha at 2 ug/mL (100 uL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL.
Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3.55-14.2 pg/mL, corresponding to a specific activity of 7.04x107~2.82x108 units/mg.