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Antibodies

HRP-conjugated Rabbit anti-Monkey IgE (Fc) monoclonal antibody

Product Sizes
100 ul
£102.00
AS098-100UL
5 x 100 ul
£258.00
AS098-5X100UL
1000 ul
£395.00
AS098-1000UL
About this Product
SKU:
AS098
Application:
ELISA
Clonality:
Monoclonal
Conjugate:
HRP
Host:
Rabbit
Immunogen:
This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Non-human Primate
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Secondary Antibodies
  • Manufacturer's Data Sheet:AS098
Dose response curve of HRP conjugated Rabbit Anti-Monkey IgE mAb measured by ELISA. 25 ng of Monkey IgE recombinant protein was coated in 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 uL of HRP conjugated Rabbit Anti-Monkey IgE mAb in a 2 fold serial dilution from 1 ug/mL to 7.8 pg/mL, incubation was performed at room temperature for 1 hour. EC50 value ( 6.54x10^-2 ug/mL) were calculated using 4 parameter logistic regression.
Cross reactivity to various IgGs of HRP conjugated Rabbit Anti-Monkey IgE mAb was examined ELISA. 1 ug/mL of various immunoglobulins were coated to 384-well plate. Then the plate was blocked and incubated with 25 uL of HRP conjugated Rabbit Anti-Monkey IgE mAb , 0.5 ug/mL and 0.125 ug/mL) at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgE antibody has highly specific recognition of -Monkey IgE, while no or minimal cross reactivity to Monkey IgD, Monkey IgA, Human IgD and Mouse IgD.