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Antibodies

HRP-conjugated Rabbit anti-Human IgD monoclonal antibody

Product Sizes
100 ul
£104.00
AS095-100UL
5 x 100 ul
£258.00
AS095-5X100UL
1000 ul
£395.00
AS095-1000UL
About this Product
SKU:
AS095
Application:
ELISA
Clonality:
Monoclonal
Conjugate:
HRP
Host:
Rabbit
Immunogen:
This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Secondary Antibodies
  • Manufacturer's Data Sheet:AS095
Dose response curve of HRP conjugated Rabbit Anti-Human IgD mAb measured by ELISA. 25 ng of Human IgD recombinant protein was coated in 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 uL of HRP conjugated Rabbit Anti-Human IgD mAb in a 2 fold serial dilution from 1 ug/mL to 7.8 pg/mL, incubation was performed at room temperature for 1 hour. EC50 value ( 2.10x10^-2 ug/mL) were calculated using 4 parameter logistic regression.
Cross reactivity to various IgGs of HRP conjugated Rabbit Anti-Human IgD mAb was examined ELISA. 1 ug/mL of various immunoglobulins were coated to 384-well plate. Then the plate was blocked and incubated with 25 uL of HRP conjugated Rabbit Anti-Human IgD mAb , 0.5 ug/mL and 0.125 ug/mL) at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgD antibody has highly specific recognition of Human IgD, while no or minimal cross reactivity to Human IgG1, Human IgG2, Human IgG3, Human IgG4, Human IgM, Human IgE and Human IgA1.