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Antibodies

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

Product Sizes
100 ul
£94.00
AS038-100UL
5 x 100 ul
£229.00
AS038-5X100UL
About this Product
SKU:
AS038
Application:
IHC-Paraffin, Western Blot
Conjugate:
HRP
Host:
Donkey
Immunogen:
This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Rabbit
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Secondary Antibodies
  • Manufacturer's Data Sheet:AS038
Western blot analysis of lysates from HeLa cells, using B Beta-actin antibody as the primary antibody. Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (AS038) at 1:2000-1:30000 dilution Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human liver (primary antibody is acox1) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver (the primary antibody is acox1, And the control secondary antibody is stained) using HRP Donkey Anti-Rabbit IgG (H+L) (AS038) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.