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Antibodies

Phospho-MKK3-S189+MKK6-S207 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP1449-20UL
100 ul
£342.00
AP1449-100UL
500 ul
£1532.00
AP1449-500UL
1000 ul
£2704.00
AP1449-1000UL
About this Product
SKU:
AP1449
Additional Names:
MAP2K3/MAP2K6|Phospho-MKK3-S189+MKK6-S207
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
36kDa/39kDa/31kDa/37kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
VDSVA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP1449
Western blot analysis of lysates from HeLa cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human breast using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-MKK3-S189+MKK6-S207 Rabbit mAb (AP1449) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.