Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Phospho-Histone H3-S28 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP1431-20UL
100 ul
£342.00
AP1431-100UL
500 ul
£1532.00
AP1431-500UL
1000 ul
£2704.00
AP1431-1000UL
About this Product
SKU:
AP1431
Additional Names:
H3/A|H3C10|H3C11|H3C12|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8|H3FA|HIST1H3A|Phospho-Histone H3-S28
Application:
ELISA, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
RKSAP
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP1431
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-S28 Rabbit mAb (AP1431) at 1:6000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with Nocodazole (100 ng/mL) at 37°C for 17 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1 s.
Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit mAb (AP1431) at 1:6000 dilution incubated overnight at 4°C. NIH/3T3 and C6 cells were treated with Nocodazole (100 ng/mL) at 37°C for 17 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20 s.
Immunoprecipitation analysis of 600 ug extracts of HeLa cells treated with Calyculin A (100nM, 30min) using 5 ug Phospho-Histone H3-S28 Rabbit mAb (AP1431). Western blot was performed from the immunoprecipitate using Phospho-Histone H3-S28 Rabbit mAb (AP1431) at a dilition of 1:2000.