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Antibodies

Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP1430-20UL
100 ul
£342.00
AP1430-100UL
500 ul
£1532.00
AP1430-500UL
1000 ul
£2704.00
AP1430-1000UL
About this Product
SKU:
AP1430
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
48KDa/51KDa/55KDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
TITPP
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP1430
Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at 1:10000 dilution. RD cells were treated by L Lambda-PP mixed solution (1ul) at 30°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of lysates from C2C12 cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at 1:10000 dilution. C2C12 cells were treated by L Lambda-PP mixed solution (1ul) at 30°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb (AP1430) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.