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Antibodies

Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP1394-20UL
100 ul
£342.00
AP1394-100UL
500 ul
£1532.00
AP1394-500UL
1000 ul
£2704.00
AP1394-1000UL
About this Product
SKU:
AP1394
Additional Names:
HDAC4/HDAC5/HDAC9|Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
57-65 kDa/97-122 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP1394
Western blot analysis of lysates from C2C12 cells using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394) at 1:1000 dilution incubated at room temperature for 1.5 hours. C2C12 treated with CIP (1 U/ul) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30 s.
Western blot analysis of lysates from HeLa cells using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394) at 1:1000 dilution incubated at room temperature for 1.5 hours. HeLa treated with L Lambdapp (2 U/ul) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60 s.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit mAb (AP1394) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.