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Antibodies

Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP1343-20UL
100 ul
£342.00
AP1343-100UL
500 ul
£1532.00
AP1343-500UL
1000 ul
£2704.00
AP1343-1000UL
About this Product
SKU:
AP1343
Additional Names:
HSPC193|HsT17436|JV15-2|LDS1C|LDS3|MADH3|Phospho-Smad2-S465/S467 + Smad3-S423/S425
Application:
ELISA, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
48kDa/52kDa/25kDa/35kDa/43kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
SSMS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP1343
Western blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (AP1343) at1:2000 dilution. HeLa and NIH/3T3 cells were treated with TGF-B Beta3 (H) (200 ng/mL) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of various lysates using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (AP1343) at 1:1000 dilution. C6 cells were treated with TGF-B Beta3 (200 ng/mL) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.