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Antibodies

Phospho-Smad3-S423/S425 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP1263-20UL
100 ul
£401.00
AP1263-100UL
About this Product
SKU:
AP1263
Additional Names:
hMAD-3|hSMAD3|HSPC193|HsT17436|JV15-2|LDS1C|LDS3|mad3|MADH3|Phospho-Smad3-S423/S425
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
48kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP1263
Western blot analysis of various lysates using (AP1263) at 1:1000 dilution. Hela cells were treated with TGF-B Beta (10 ng/ml) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Western blot analysis of various lysates using (AP1263) at 1:1000 dilution. C2C12 cells were treated with TGF-B Beta (10 ng/ml) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.