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Antibodies

Phospho-Vimentin-S83 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP1119-20UL
100 ul
£401.00
AP1119-100UL
About this Product
SKU:
AP1119
Additional Names:
CTRCT30|HEL113|Phospho-Vimentin-S83|VIM|vimentin
Application:
ELISA, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
54kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
QDSVD
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP1119
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (AP1119) at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (100 nM/ml) at 37°C for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from C6 cells, using Phospho-Vimentin-S83 Rabbit pAb (AP1119) at 1:1000 dilution. C6 cells were treated with Paclitaxel (100 nM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 Rabbit pAb (AP1119) at 1:1000 dilution. HeLa cells were treated with Paclitaxel (100 nM/ml) at 37°C for 20 hours. HeLa cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.