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Antibodies

Phospho-HSP27/HSPB1-S82 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP1031-20UL
100 ul
£342.00
AP1031-100UL
500 ul
£1532.00
AP1031-500UL
1000 ul
£2704.00
AP1031-1000UL
About this Product
SKU:
AP1031
Additional Names:
CMT2F|HEL-S-102|HMN2B|HS.76067|Hsp25|HSP27|HSP28|Phospho-HSP27/HSPB1-S82|SRP27
Application:
ELISA, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
23 kda
Purification:
Affinity Purified
Reactivities:
Human, Rat
Sequence:
QLSSG
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP1031
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031) at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Western blot analysis of lysates from PC-12 cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (AP1031) at 1:1000 dilution. PC-12 cells were treated with CIP (1 U/ul) at 37°C for 1 hour or treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.