Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Phospho-POLR2A CTD-S5 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP0997-20UL
100 ul
£342.00
AP0997-100UL
500 ul
£1532.00
AP0997-500UL
1000 ul
£2704.00
AP0997-1000UL
About this Product
SKU:
AP0997
Additional Names:
hRPB220|hsRPB1|NEDHIB|Phospho-POLR2A CTD-S5|POLR2|POLRA|RPB1|RPBh1|RpIILS|RPO2|RPOL2
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
217kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP0997
Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at 1:1000 dilution. MCF7 cells were treated with Doxorubicin (0. 5 uM) at 37°C for 24 hours or treated with nocodazole (50 ng/mL) at 37°C for 20 hours or treated with CIP(20uL/400ul) at 37°C for 1 hour. C2C12 cells were treated with CIP(20uL/400ul) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.