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Antibodies

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP0974-20UL
100 ul
£342.00
AP0974-100UL
500 ul
£1532.00
AP0974-500UL
1000 ul
£2704.00
AP0974-1000UL
About this Product
SKU:
AP0974
Additional Names:
ERK-1|ERK1|ERT2|HS44KDAP|HUMKER1A|p44-ERK1|p44-MAPK|P44ERK1|P44MAPK|Phospho-ERK1-T202/Y204 + ERK2-T185/Y187|PRKM3
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
42kDa/44kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
LTEYV
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP0974
Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4°C. 293T cells were treated with PMA (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4°C. C6 cells were treated with PMA (200 nM) at 37°C for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.