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Antibodies

Phospho-POLR2A CTD-S7 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0969-20UL
100 ul
£401.00
AP0969-100UL
About this Product
SKU:
AP0969
Additional Names:
hRPB220|hsRPB1|NEDHIB|Phospho-POLR2A CTD-S7|POLR2|POLRA|RPB1|RPBh1|RpIILS|RPO2|RPOL2
Application:
ChIP, ELISA, IHC-Paraffin, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
217kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
SPSYS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0969
Western blot analysis of lysates from MCF7 cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at 1:1000 dilution. MCF7 cells were treated with Doxorubicin (0. 5 uM) at 37°C for 24 hours. MCF7 cells were treated with nocodazole (50 ng/ml) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.