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Antibodies

Phospho-p53-S392 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0860-20UL
100 ul
£401.00
AP0860-100UL
About this Product
SKU:
AP0860
Additional Names:
BCC7|BMFS5|LFS1|P53|Phospho-p53-S392|TRP53
Application:
ELISA, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
44kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
GPDSD
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C 50% glycerol. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0860
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S392 Rabbit pAb (AP0860) at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (100 nM/ml) at 37°C for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
Western blot analysis of various lysates using Phospho-p53-S392 Rabbit pAb (AP0860) at 1:1000 dilution. HeLa cells were treated with Paclitaxel (100 nM/ml) at 37°C for 20 hours. HeLa cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours. C6 cells were treated with Paclitaxel (100 nM) at 37°C for 20 hours. C6 cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s.