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Antibodies

Phospho-Histone H3-S28 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0839-20UL
100 ul
£401.00
AP0839-100UL
About this Product
SKU:
AP0839
Additional Names:
H3/A|H3C10|H3C11|H3C12|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8|H3FA|HIST1H3A|Phospho-Histone H3-S28
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
ARKSA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0839
Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution. C6 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s.
Immunofluorescence analysis of H9C2 cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.