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Antibodies

Phospho-Lamin A/C-S22 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0777-20UL
100 ul
£401.00
AP0777-100UL
About this Product
SKU:
AP0777
Additional Names:
CDCD1|CDDC|CMD1A|CMT2B1|EMD2|FPL|FPLD|FPLD2|HGPS|IDC|LDP1|LFP|LGMD1B|LMN1|LMNC|LMNL1|MADA|Phospho-Lamin A/C-S22|PRO1
Application:
ELISA, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
74kDa
Purification:
Affinity Purified
Reactivities:
Human, Rat
Sequence:
PLSPT
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C 50% glycerol. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0777
Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (AP0777) at 1:2000 dilution or Lamin A antibody (A0249). HeLa cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours or treated with Paclitaxel (100 nM/mL) at 37°C for 20 hours. C6 cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours or treated with Paclitaxel (100 nM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug Phospho-Lamin A/C-S22 pAb (AP0777). Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb (AP0777) at a dilution of 1:1000. HeLa cells were treated with Paclitaxel (100 nM/mL) at 37°C for 20 hours.