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Antibodies

Phospho-POLR2A CTD-S2 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0749-20UL
100 ul
£401.00
AP0749-100UL
About this Product
SKU:
AP0749
Additional Names:
hRPB220|hsRPB1|NEDHIB|Phospho-POLR2A CTD-S2|POLR2|POLRA|RPB1|RPBh1|RpIILS|RPO2|RPOL2
Application:
ELISA, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
217kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
SYSPT
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C 50% glycerol. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0749
Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (AP0749) at 1:1000 dilution. 293T cell lysate were treated with CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated with Doxorubicin (0.5uM) for 24 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (AP0749) at 1:1000 dilution or POLR2A antibody (A2107). 293T cells were treated with CIP(20uL/400ul) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug Phospho-POLR2A CTD-S2 antibody (AP0749). Western blot was performed from the immunoprecipitate using Phospho-POLR2A-S2 pAb (AP0749) at a dilution of 1:1000. MCF7 cells were treated with nocodazole (50 ng/mL) at 37°C for 20 hours.