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Antibodies

Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0548-20UL
100 ul
£401.00
AP0548-100UL
About this Product
SKU:
AP0548
Additional Names:
HSPC193|HsT17436|JV15-2|LDS1C|LDS3|MADH3|Phospho-Smad2-S465/467 + Smad3-S423/425
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
48kDa/52kDa/25kDa/35kDa/43kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
RCSSMS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0548
Western blot analysis of lysates from C2C12 cells, using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at 1:800 dilution. C2C12 cells were treated with TGF-B Beta (10 ng/ml) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse stomach using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.