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Antibodies

Phospho-p38 MAPK-T180/Y182 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0526-20UL
100 ul
£401.00
AP0526-100UL
About this Product
SKU:
AP0526
Additional Names:
CSBP|CSBP1|CSBP2|CSPB1|EXIP|MAPK14|Mxi2|p38|p38 MAPK|p38ALPHA|Phospho-p38 MAPK-T180/Y182|PRKM14|PRKM15|RK|SAPK2A
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
29kDa/34kDa/35kDa/41kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MTGYV
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0526
Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at 1:18000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at 1:18000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at a dilution of 1:250 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-p38 MAPK-T180/Y182 Rabbit pAb (AP0526) at a dilution of 1:250 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.