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Antibodies

Phospho-p70 S6 Kinase 1-T421/S424 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP0502-20UL
100 ul
£342.00
AP0502-100UL
500 ul
£1532.00
AP0502-500UL
1000 ul
£2704.00
AP0502-1000UL
About this Product
SKU:
AP0502
Additional Names:
p70 S6KA|p70-alpha|p70-S6K|p70(S6K)-alpha|Phospho-p70 S6 Kinase 1-T421/S424|PS6K|S6K|S6K-beta-1|S6K1|STK14A
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
59kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
SVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP0502
Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502) at 1:1000 dilution. NIH/3T3 and C6 cells were treated with 10% FBS at 37°C for 30 minutes after serum-starvation overnight Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60S.
Confocal imaging of HeLa cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of C6 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.