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Antibodies

Phospho-YAP1-S127 Rabbit polyclonal antibody

Product Sizes
20 ul
£151.00
AP0489-20UL
100 ul
£336.00
AP0489-100UL
500 ul
£1532.00
AP0489-500UL
1000 ul
£2704.00
AP0489-1000UL
About this Product
SKU:
AP0489
Additional Names:
COB1|Phospho-YAP1-S127|YAP|YAP-1|YAP2|YAP65|YKI
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
54kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0489
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-YAP1-S127 Rabbit pAb (AP0489) at 1:400 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.