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Antibodies

Phospho-NF-kB p65/RelA-S536 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0475-20UL
100 ul
£401.00
AP0475-100UL
About this Product
SKU:
AP0475
Additional Names:
AIF3BL3|CMCU|NFKB3|p65|Phospho-NF-kB p65/RelA-S536
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
60kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
FSSIA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0475
Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:17000 dilution incubated overnight at 4°C. HeLa cells were treated with CA (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.