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Antibodies

Phospho-SHP2-Y542 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0267-20UL
100 ul
£401.00
AP0267-100UL
About this Product
SKU:
AP0267
Additional Names:
BPTP3|CFC|JMML|METCDS|NS1|Phospho-SHP2-Y542|PTP-1D|PTP2C|SH-PTP2|SH-PTP3|SHP2
Application:
ELISA, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
68kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
HEYTN
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0267
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SHP2-Y542 Rabbit pAb (AP0267) at 1:700 dilution. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of lysates from C6 cells, using Phospho-SHP2-Y542 Rabbit pAb (AP0267) at 1:700 dilution. C6 cells were treated with PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.