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Antibodies

Phospho-ERK1-Y204 Rabbit polyclonal antibody

Product Sizes
20 ul
£169.00
AP0235-20UL
100 ul
£401.00
AP0235-100UL
About this Product
SKU:
AP0235
Additional Names:
ERK-1|ERK1|ERT2|HS44KDAP|HUMKER1A|p44-ERK1|p44-MAPK|P44ERK1|P44MAPK|Phospho-ERK1-Y204|PRKM3
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
43kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
TEYVA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:AP0235
Western blot analysis of lysates from NIH/3T3 cells using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at 1:1000 dilution incubated overnight at 4°C. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human uterus usingPhospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using Phospho-ERK1-Y204 Rabbit pAb (AP0235) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of methanol-fixed HeLa cells showing centrosome and nuclear staining using Phospho-ERK1-Y204 Rabbit pAb (AP0235).