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Antibodies

Phospho-Histone H3-S10 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
AP0002-20UL
100 ul
£342.00
AP0002-100UL
500 ul
£1532.00
AP0002-500UL
1000 ul
£2704.00
AP0002-1000UL
About this Product
SKU:
AP0002
Additional Names:
H3/A|H3C10|H3C11|H3C12|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8|H3FA|HIST1H3A|Phospho-Histone H3-S10
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
ARKST
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AP0002
Western blot analysis of lysates from HeLa,NIH/3T3,C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at 1:1000 dilution. HeLa,NIH/3T3 and C6 cells were treated with nocodazole (50 ng/mL) at 37°C for 20 hours or treated with CIP(20uL/400ul) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Confocal imaging of NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of HeLa cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunohistochemistry analysis of paraffin-embedded Human oophoroma using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.