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Antibodies

HA-Tag Rabbit monoclonal antibody

Product Sizes
50 ul
£145.00
AE105-50UL
100 ul
£205.00
AE105-100UL
200 ul
£318.00
AE105-200UL
500 ul
£639.00
AE105-500UL
1000 ul
£1151.00
AE105-1000UL
About this Product
SKU:
AE105
Additional Names:
HA|HA tag|HA-tag
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Wide/Non-species Specific
Sequence:
YPYDVPDYA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE105
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NFAT-HA-Tag using HA-Tag Rabbit mAb (AE105) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 10s.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with LDHA-HA-Tag using HA-Tag Rabbit mAb (AE105) at 1:40000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 10s.
Immunofluorescence analysis of 293T-SCRNI-HA-GFP(C) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293T-SCRNI-HA-GFP(N) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.