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Antibodies

DDDDK-Tag Rabbit monoclonal antibody

Product Sizes
50 ul
£145.00
AE092-50UL
100 ul
£205.00
AE092-100UL
200 ul
£318.00
AE092-200UL
500 ul
£639.00
AE092-500UL
1000 ul
£1151.00
AE092-1000UL
About this Product
SKU:
AE092
Additional Names:
DDDDK|DDDDK tag|DDDDK-tag
Application:
ChIP, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Wide/Non-species Specific
Sequence:
DYKDDDDK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE092
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GSK3B Protein,BCAT2-N Protein,BCAT2-C Protein, using DDDDK-Tag antibody (AE092) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from wild type (WT),293F transfected with COPB2-N Protein,COPB2-C Protein,using DDDDK-Tag antibody (AE092) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Chromatin immunoprecipitation was performed with 21.8 ug of cross-linked chromatin from 293T cells transfected with a GATA3 expression vector containing a single C-terminal DDDDK-Tag using 5 ug of Rabbit anti DDDDK-Tag (AE092). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DDDDK-Tag across chromosome 2 (upper panel) and the genomic region encompassing SH3RF3, a representative gene enriched in DDDDK-Tag (lower panel).
Chromatin immunoprecipitation was performed with 21.8 ug of cross-linked chromatin from 293T cells transfected with a GATA3 expression vector containing a single C-terminal DDDDK-Tag using 5 ug of Rabbit anti DDDDK-Tag (AE092). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of DDDDK-Tag in the representative genomic region surrounding SH3RF3 gene.
Confocal imaging of 293T cells transfected with GFP-DDDDK-Tag (N) and 293T cells transfected with GFP-DDDDK-Tag (C) cells using DDDDK-Tag Rabbit mAb (AE092, dilution 1:300) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.