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Antibodies

Rabbit anti His-Tag polyclonal antibody

Product Sizes
50 ul
£127.00
AE068-50UL
100 ug
£181.00
AE068-100UG
200 ul
£270.00
AE068-200UL
500 ul
£532.00
AE068-500UL
1000 ul
£948.00
AE068-1000UL
About this Product
SKU:
AE068
Additional Names:
His|His tag|His-tag
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Label/Epitope-tag, Wide/Non-species Specific
Sequence:
HHHHHH
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE068
Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using Rabbit anti His-Tag pAb (AE068) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-Tag with 3 ug Rabbit anti His-Tag pAb antibody (AE068).Western blot was performed from the immunoprecipitate using Rabbit anti His-Tag pAb antibody (AE068) at 1:5000 dilution.
Immunoprecipitation analysis of extracts of cells using His-Tag pAb (AE068). Western blot was performed from the immunoprecipitate using His-Tag pAb (AE068) at a dilition of 1:3000.