Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Rabbit anti GFP-Tag polyclonal antibody

Product Sizes
50 ul
AE011-50UL
100 ul
AE011-100UL
200 ul
AE011-200UL
500 ul
AE011-500UL
1000 ul
AE011-1000UL
About this Product
SKU:
AE011
Additional Names:
GFP|GFP tag|GFP-tag
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
27kDa
Purification:
Affinity Purified
Reactivities:
Label/Epitope-tag, Wide/Non-species Specific
Sequence:
MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE011
Western blot analysis of extracts of normal 293T cells and 293T transfected with GFP-tagged fusion protein,using Rabbit anti GFP-Tag pAb (AE011) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of extracts of normal Eukaryotic expression of GFP and Eukaryotic expression of GFP transfected with GFP Protein, using Rabbit anti GFP-Tag pAb (AE011) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunofluorescence analysis of 293T and 293T-GFP cells using Rabbit anti GFP-Tag pAb (AE011) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.